Enzymatic Synthesis of Glutamine in Higher Plants

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Enzymatic Synthesis of Glutamine in Higher Plants.

The synthesis of glutamine has been studied extensively with enzyme preparations from animal tissues and from bacteria (1, 2, 3, 9, 10). These studies have shown that in such organisms, glutamine may be formed directly from glutamic acid and ammonia, the energy for the synthesis being supplied as adenosine triphosphate (ATP). Little is known, however, of the occurrence or characteristics of sim...

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Enzymatic Synthesis of Gamma-Glutamyl-Cysteine in Higher Plants.

It therefore seemed possible that the synthesis of dipeptides involving the glutamyl moiety might occur by an analogous reaction. The glutamyl peptide, gamma-glutamyl-cysteinyl-glycine (glutathione), is known to occur generally in plants. The enzymatic synthesis of gamma-glutamyl-cysteine has therefore been first investigated since this compound constitutes a possible intermediate in the synthe...

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Studies on the Enzymatic Synthesis of Glutamine.

Theorell (1942) has reported sedimentation and diffusion measurements on peroxidase: our value for S20 (corr.) is about 10% lower than his (3-85 x 10-13) (cf. Cecil & Ogston, 1948), while our value for D20 (corr.) agrees fairly well with his value of 6 84 x 1o-7. Our value for the molecular weight is lower than Theorell's (44,100), but is in agreement with that calculated by Keilin & Hartree (1...

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The enzymatic synthesis of glutamine, a reaction utilizing adenosine triphosphate.

The preceding paper (2) describes experiments on the synthesis of glutamine in pigeon liver dispersions, which, together with the results of Krebs and his collaborators (3, 4) and of Bujard and Leuthardt (5), suggested that adenosine triphosphate may play an essential r81e in amide formation. Proof for this function of adenosine triphosphate has been obt.ained in studies with tissue extracts, w...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1953

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.28.4.724